A certain metabolic pathway takes the following sequence.

A certain metabolic pathway takes the following sequence.

At the start of the experiment an inhibitor was added to the reactants. After the experiment it was found out that there was the same concentration of J, more than normal concentration of K, near absence of L, M and N. When L was added to the inhibitor set M and N were detected.

a) At what stage of the reaction sequence did the inhibitor have its effect?  (1mk)
b) Explain how the inhibitor affected the reaction.  (1mk)
c) What is the identity of substance L?  (1mk)

ANSWERS

a) At K/ between K and L
b) The inhibitor blocked the active sites of enzymes; that converts K to L; hence accumulation of K and near absence of  L,M and N;
c) Enzyme activator;